Coding

Part:BBa_K5201001:Design

Designed by: YAU LI, Shun Lam Wong   Group: iGEM24_HongKong-UCCKE   (2024-09-30)


kfiD codons are optimized for overexpression of UDP-glucose-6-dehyrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 200
    Illegal BamHI site found at 732
    Illegal XhoI site found at 1075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

kfiD is designed to optimize over-expression for E. coli. This ensures a high amount of UDP-glucuronic acid, and hence, HA can be produced.


Source

E. coli K5 strain

References